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目的 北京结核分支杆菌分子流行病学的研究。方法 (1)使用标准化的RFLP法检测并分析从 12 8位初治患者分离的结核分支杆菌IS6 110指纹多态性 ;(2 )对选例患者进行社会人口学和流行病学调查问卷 ;(3)分析结核分支杆菌IS6 110指纹多态性特征与流行病学的关系。结果 (1)根据IS6 110指纹特征的同源性 ,检测的 12 8株结核分支杆菌可基本分为 3组 ,A、B组的菌株间因具有较高的同源性而被称为“北京基因型”菌株 ,并且此类菌株在检测菌株中所占的比例较高 (10 3/ 12 8,80 .5% ) ;C组菌株间同源性较低 ,多态性变化多而被称为“非北京基因型” ,仅占 19.5%。 (2 )从较低年龄 (<4 0岁 )患者分离菌株的多态性变化 ,与从较高年龄 (>4 0岁 )患者分离菌株多态性变化比较存在差异 ,提示“北京基因型”菌株不是由于BCG接种而造成的选择性优势。 (3)检测菌株的多态性变化与患者结核病接触史、患者长期居住地、菌株药物敏感性情况无明显联系。结论 结核分支杆菌的指纹分析对结核病现代流行病学的研究是一个有用的工具。
Objective To study the molecular epidemiology of Mycobacterium tuberculosis in Beijing. Methods (1) The standardized RFLP method was used to detect and analyze the fingerprinting of Mycobacterium tuberculosis IS6 110 isolated from 12 8 untreated patients; (2) the questionnaire on social demography and epidemiology of selected patients; ( 3) To analyze the relationship between fingerprinting polymorphism of Mycobacterium tuberculosis IS6 110 and epidemiology. Results (1) According to the homology of fingerprint characteristics of IS6 110, 12 8 Mycobacterium tuberculosis detected could be divided into 3 groups basically. The strains A and B were called “Beijing Genotype ”strains, and these strains in the test strains accounted for a higher proportion (103/12 8,80 .5%); C group strains with low homology, polymorphism and more known as For non-Beijing genotype, only 19.5%. (2) The polymorphism of isolates from patients with lower age (<40 years) was significantly different from that of isolates from patients of higher age (> 40 years old), suggesting that “Beijing genotype” Strain is not a selective advantage due to BCG inoculation. (3) There was no significant correlation between the changes of the polymorphisms of the tested strains and the history of the patients with TB, the long-term residence of the patients and the drug susceptibility of the strains. Conclusion The fingerprinting analysis of Mycobacterium tuberculosis is a useful tool for the study of modern epidemiology of tuberculosis.