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在《MBNL》第16期(1980年7月)上,我们论述了“胚乳体模式”(Spermosphere Model),在这一系统中,一株幼苗在试管中于黑暗下生长,管内含矿质培养基,但没有结合态氮,唯一的碳源和能量是胚乳。通过乙炔还原作用测定联合固氮细菌固氮酶活性。该系统在快速、简便、经济地比较不同基因型间根际细菌固氮潜力方面是有价值的。 1981年,我们增加了CO_2阱,以改善试验装置,同时应用了从水稻根际分离得到的不能
In “MBNL” issue 16 (July 1980), we discuss the “Spermosphere Model” in which one seedling is grown in darkness in a test tube containing mineral medium , But without bound nitrogen, the only source of carbon and energy is the endosperm. Nitrogenase activity of combined nitrogen-fixing bacteria was determined by acetylene reduction. The system is valuable for quick, easy and economical comparison of rhizospheric bacterial nitrogen fixation potential among different genotypes. In 1981, we added CO 2 traps to improve the experimental setup, and at the same time we applied the non-ability to separate from rice rhizosphere