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目的：制备抗ｔＰＡ单克隆抗体，用于建立ＥＬＩＳＡ方法和研究ｔＰＡ与肿瘤转移的关系。方法：用基因重组的组织型纤溶酶原激活剂（ｒｔＰＡ）免疫Ｂａｌｂ／ｃ小鼠，按常规法作细胞融合，用ＥＬＩＳＡ法筛选出阳性反应的杂交瘤细胞克隆。结果：获得一株杂交瘤细胞株（２Ｂ７）；其免疫球蛋白亚类为ＩｇＧ１；经ＥＬＩＳＡ法和免疫印迹技术证明，此单抗能特异性识别ｔＰＡ。结论：用２Ｂ７ＭｃＡｂ特异性强，用其建立的夹心ＥＬＩＳＡ检测ｔＰＡ的方法线性较好，灵敏度可达１ｎｇ／ｍｌ。２Ｂ７ＭｃＡｂ可望用于ｔＰＡ与肿瘤转移关系及导向溶栓的研究。
Objective: To prepare anti-tPA monoclonal antibody for the establishment of ELISA method and study the relationship between tPA and tumor metastasis. METHODS: Balb/c mice were immunized with recombinant tissue-type plasminogen activator (rtPA), and cell fusion was performed according to conventional methods. Positive hybridoma cell clones were screened by ELISA. RESULTS: A hybridoma cell line (2B7) was obtained. Its immunoglobulin subclass was IgG1. The ELISA and immunoblot techniques demonstrated that this monoclonal antibody specifically recognized tPA. Conclusion: The specificity of 2B7McAb is strong, and the sandwich ELISA established with it has a good linearity and sensitivity of 1ng/ml. 2B7McAb is expected to be used for the study of the relationship between tPA and tumor metastasis, and for the study of directional thrombolysis.