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目的:以等基线多波长覆盖融合技术为基础的红花多成分含量测定方法,对红花药材进行质量控制。方法:采用高效液相色谱法,Agilent TC-C18(4.6mm×250mm,5μm)色谱柱,流动相为0.4%磷酸水-乙腈线性梯度洗脱,流速1.0mL/min;柱温25℃;DAD检测器波长为327nm、360nm和380nm,使用Matlab软件编程,对三个波长下的dif格式数据进行等基线多波长覆盖融合。结果:本方法各主要成分色谱峰之间有良好的分离度,羟基红花黄色素A、咖啡酸、芦丁和山奈酚分别在1.007～1.922μg/mL、0.0551～0.0772μg/mL、0.133～0.253μg/mL、0.0342～0.0652μg/mL范围内呈良好的线性关系。结论:该方法简便、合理、可靠、重复性好,可用于红花药材的质量评价。
OBJECTIVE: To determine the content of safflower multi-constituents based on the same baseline multi-wavelength coverage fusion technology and to control the quality of safflower medicinal materials. Methods: High performance liquid chromatography (HPLC) was performed on a Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of 0.4% phosphoric acid in water and acetonitrile linear gradient at a flow rate of 1.0 mL / The detector wavelength is 327nm, 360nm and 380nm. Using Matlab software programming, the iso-format data at three wavelengths are covered by the same baseline multi-wavelength coverage. Results: There was a good resolution between the chromatographic peaks of the main components of this method. The concentrations of hydroxysafflor yellow A, caffeic acid, rutin and kaempferol were 1.007 ~ 1.922μg / mL, 0.0551 ~ 0.0772μg / mL and 0.133 ~ 0.253 μg / mL, 0.0342 ~ 0.0652μg / mL showed a good linear relationship. Conclusion: The method is simple, reasonable, reliable and reproducible. It can be used for the quality evaluation of safflower medicinal materials.