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背景:脑出血是中枢神经系统非病原微生物感染机制致病的极严重的损伤事件,神经-内分泌-免疫系统调节网络的功能活动必然十分明显,内啡肽是调节网络中重要的系统间调节信号,通过探讨疾病过程中内源性β内啡肽水平的变化与免疫系统功能的关系,以及外源性β内啡肽对体外培养的免疫活性细胞功能的影响,能够进一步了解神经内分泌免疫调节网络的作用机制,为可能进行的临床干预治疗及康复措施介入提供实验依据。目的:探讨脑出血患者免疫系统功能的变化及内源性β内啡肽与疾病的关系。设计:病例-对照研究。单位:首都医科大学附属复兴医院神经内科,北京市红十字会急救中心,中国协和医科大学的基础医学研究所免疫室。对象:2001-12/2002-06北京复兴医院和红十字急救中心就诊的部分脑出血患者28例(脑出血组)。2002-02/06北京复兴医院住院的部分恢复期脑缺血患者28例(脑缺血对照组)。2002-02/06北京协和医院正常体检人群28例(正常对照组)。方法:应用放射免疫分析法检测外周血β内啡肽含量;用RT-PCR半定量分析方法检测了外周血单个核细胞白细胞介素(IL)-1β,IL-2,IL-8和一氧化氮合酶(iNOS)mRNA表达;β内啡肽对体外培养的脑出血患者外周血单个核细胞的IL-1β,IL-2,IL-8和iNOS表达的影响。主要观察指标:①外周血β内啡肽
BACKGROUND: Intracerebral hemorrhage is a very serious injury event caused by the pathogenesis of non-pathogenic microorganisms in the central nervous system. The functional activity of the neuro-endocrine-immune system regulatory network is bound to be obvious. Endorphins are important intersystem regulatory signals in the regulatory network , By exploring the relationship between endogenous β-endorphin levels and immune system function during the disease process and the effect of exogenous β-endorphin on the function of immunocompetent cells cultured in vitro, we can further understand the neuroendocrine immune regulatory network Mechanism of action for the possible clinical interventions and rehabilitation intervention intervention to provide experimental evidence. Objective: To investigate the changes of immune system in patients with intracerebral hemorrhage and the relationship between endogenous β-endorphin and diseases. Design: Case-control study. SETTING: Department of Neurology, Renaissance Hospital, Capital Medical University, Beijing Red Cross Emergency Center, Peking Union Medical College, Institute of Basic Medical Sciences. PARTICIPANTS: A total of 28 patients with cerebral hemorrhage (ICH) treated by Beijing Fuxing Hospital and Red Cross Emergency Center from December 2001 to June 2002 were enrolled. 2002-02 / 06 Beijing Fuxing Hospital inpatient partial recovery of 28 patients with cerebral ischemia (cerebral ischemia control group). 2002-02 / 06 Beijing Union Medical College Hospital 28 normal checkups (normal control group). Methods: The content of β-endorphin in peripheral blood was detected by radioimmunoassay. The levels of IL-1β, IL-2 and IL-8 in peripheral blood mononuclear cells were detected by semi-quantitative RT-PCR. Effects of β-endorphin on the expression of IL-1β, IL-2, IL-8 and iNOS in peripheral blood mononuclear cells in patients with intracerebral hemorrhage. MAIN OUTCOME MEASURES: ① Peripheral blood β endorphin