目的:进行一个前瞻性、多中心的研究,来评估实验室证实的流感对健康儿童及其家庭产生的负担。方法:由5名初级医疗的儿科医师(PCPs)每周抽出两天时间登记来自意大利不同大城市的4个急诊科(EDs)在2001年11月1日至2002年4月30日期间所治疗的患儿。3771名14岁以下没有慢性疾病的儿童参与了该研究,这些儿童是研究期间在EDs或PCP门诊诊断为呼吸道感染的患儿。搜集患儿的鼻咽部拭子进行流感病毒分离及其RNA检测。同时搜集患儿及与其有接触的 Purpose: To conduct a prospective, multicenter study to assess laboratory-generated burden of influenza on healthy children and their families. METHODS: Five primary-care pediatricians (PCPs) were given two days a week to register four EDs from different large Italian cities for treatment between November 1, 2001 and April 30, 2002 Children. A total of 3,771 children under the age of 14 who did not have a chronic disease were included in the study, who were diagnosed as having respiratory tract infections at EDs or PCP clinics during the study period. Nasopharyngeal swabs were collected for influenza virus isolation and RNA detection. At the same time collect children and have contact with them