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目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法 :用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫 Cytc1基因及其部分序列完全相同。
Objective: To determine and compare the partial sequences of NADH1 and Cytc1 genes in Zhejiang strain and Anhui strain of Schistosoma japonicum. Methods: NADH1 and Cytc1 DNA genes were amplified by PCR from specific primers and then sequenced. Results: Two adult schistosoma japonicum NADH1 and Cytc1 genes and their sequences were obtained. Conclusion: The NADH1 gene and its partial sequence of adult Schistosoma japonicum are identical except for nucleotide 382 (C / T). The two strains of Schistosoma japonicum Cytc1 gene and its partial sequence are exactly the same.