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通过了人体断层皮片冰冻切片及分离所得的表皮层作ATP酶染色,显示出郎罕(Langerhans)细胞位于基底细胞之上,呈树突状分支,细胞之间的分支可相互交错呈网状结构。在表皮细胞(S)悬液中,应用EAIgG与郎罕细胞结合成花环,花环形成率为3.24±0.90%,并通过密度梯度分离,得到了富有郎罕细胞的表皮细胞(Se)和缺乏郎罕细胞的表皮细胞(Sd)悬液。把S、Se和Sd同时与另一个体的淋巴细胞作混合培养,20组实验的平均刺激指数为:MSLC=54.87±28.3;MSeLC=49.54±32.72;MSdLC=20.84±14.89。MSLC和MSeLC都明显高于MSdLC(P<0.01)。由于特异的Ia抗血清和补体能阻断MSLC和MSeLC反应,说明了在MSLC中,由于郎罕细胞的存在引起了淋巴细胞的增殖反应。本实验为临床上异体与自体皮肤混合移植后,异体表皮的排异先于异体真皮的现象,进一步提供了理论根据。
The frozen sections of the human skin slices and the separation of the resulting epidermal layer by ATPase staining showed that Langerhans cells are located above the basal cells with dendritic branches and the branches between the cells can be interlaced with each other structure. In the epidermal cells (S) suspension, the combination of EAIgG and Langerhans cells into a rosette, rosette formation rate was 3.24 ± 0.90%, and separated by density gradient to obtain rich Lan Lan cells of epidermal cells (Se) and the lack of Lang Rare cell epidermal cells (Sd) suspension. S, Se and Sd at the same time with another body of lymphocytes were mixed culture, 20 groups of experiments with the average stimulation index: MSLC = 54.87 ± 28.3; MSeLC = 49.54 ± 32.72; MSdLC = 20.84 ± 14.89. MSLC and MSeLC were significantly higher than MSdLC (P <0.01). Since specific Ia antisera and complement can block the MSLC and MSeLC reactions, it is demonstrated that in MSLC, lymphocyte proliferation reaction is caused by the presence of Langerhans cells. This experiment for clinical heterogeneous mixed with autologous skin transplantation, allogeneic epidermal abnormalities in allogeneic dermis prior to further provide a theoretical basis.