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本文用 1.灵芝子实体经水提、乙醇沉制得灵芝多糖 (GL PS)精品 ,用苯酚 -硫酸法测其百分含量。 2 .应用小鼠移植性恶性肿瘤瘤株 (S1 80 、U1 4 、EAC) ,观察 GL PS对荷瘤小鼠肿瘤生长的影响。 3.观察 GL PS对小鼠全血凝血时间及出血时间和大鼠血栓及血液凝固系统的影响。实验结果 1.用本方法提取 GL PS得率为 0 .5 89± 0 .135 %。2 .GL PS 40 m g/ (kg· d) .ig× 17d对小鼠肉瘤 (S- 180 )和宫颈癌 (U - 14)的平均抑瘤率分别为 43%和 5 1% ,而对艾氏腹水癌 (EAC)小鼠生命没有明显延长作用 (P>0 .0 5 )。 3.GL PS 2 0 m g/ (kg· d) .ig× 10 d或 2 0～ 40 mg· kg- 1 .iv均能明显延长小鼠的凝血时间和出血时间 (P<0 .0 1)。GL PS 40 m g/ (kg· d) .ig× 10 d能明显抑制大鼠的血栓形成 (P<0 .0 1) ,降低纤维蛋白原的含量 (P<0 .0 1) ,同时能明显延长部分凝血活酶时间 (KPTT) (P<0 .0 1)。说明 GL PS具有抗肿瘤、抗血栓和抗凝血的作用
This article uses 1. Ganoderma lucidum fruiting body by water extraction, ethanol precipitation Ganoderma lucidum polysaccharide (GL PS) fine, using phenol - sulfuric acid method to determine its percentage. 2. To observe the effect of GL PS on the tumor growth of tumor-bearing mice by using mouse transplantable tumor tumor strains (S1 80, U14, EAC). 3. Observe the effect of GL PS on the clotting time and bleeding time of mice and the thrombosis and blood coagulation system in mice. Experimental results 1. The extraction rate of GL PS by this method is 0.59 ± 0.135 %. 2. The average tumor inhibition rates of mouse sarcoma (S-180) and cervical cancer (U-14) were 43% and 51%, respectively, for GL PS 40 mg/(kg·d) and .ig×17d. There was no significant prolongation of life in E. coli mice (P > 0.05). 3. GL PS 2 0 mg/ (kg·d)·ig×10 d or 20~40 mg·kg- 1·iv all significantly prolonged clotting time and bleeding time in mice (P<0.01). . GL PS 40 mg/ (kg·d)·ig×10 d significantly inhibited thrombosis in rats (P<0.01), decreased fibrinogen levels (P<0.01), and significantly Increased partial thromboplastin time (KPTT) (P < 0.01). Description GL PS has anti-tumor, anti-thrombotic and anti-coagulant effects