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用RT－PCR的方法从非甲、乙、丙、丁、戊型肝炎病人血清中检测到一株庚型肝炎病毒（GBV－C/HGV）。其中扩增NS3区基因采用56个循环周期的减温PCR方法；扩增NS5区基因采用35个循环周期的正常PCR方法。并对其PCR产物进行了克隆测序。在NS3区，样品与GBV－C和HGV的核苷酸序列同源性分别为84．2％和89．9％；而在NS5区样品与HBV－V和HGV的核苷酸同源性分别为94．9％和98．1％，因此，NS3区的基因变异似乎大于NS5区的变异，而且这种变异可能与HCV的基因变异趋势类似。
A hepatitis G virus (GBV-C / HGV) was detected by RT-PCR from the sera of non-A, B, C, D and E hepatitis patients. The amplification of NS3 region genes using 56 cycles of temperature reduction PCR method; amplified NS5 gene using 35 cycles of normal PCR method. The PCR product was cloned and sequenced. In the NS3 region, the nucleotide sequence homologies of the samples to GBV-C and HGV were 84.2% and 89.9%, respectively; whereas in the NS5 region, nucleotide homology to HBV-V and HGV was 94.9% and 98.1% respectively. Therefore, the gene mutation in NS3 region seems to be larger than that in NS5 region, and this variation may be similar to the tendency of HCV gene mutation.