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目的研究早熟凝集染色体(PCC)方法学的常规法和改进后的方法对60Coγ射线剂量-效应曲线影响,为事故应急选择快速和准确的生物剂量估算方法。方法取3名23～28岁的男性肘静脉血,在60Coγ射线照射(01、.0、5.0、10.0、15.02、0.0 Gy,吸收剂量率0.635 Gy/min)的条件下,以常规PCC培养方法为对比;比较2种方法(常规法和改进法)的50 h和60 h的2个培养时间所产生剂量-效应曲线的关系;以另一实验室照射10.0 Gy(吸收剂量率0.670 Gy/min)所产生的PCC环结果,用上述制作的剂量-曲线估算剂量作验证。结果①常规50 h培养方法15.0、20.0 Gy点所获PCC细胞数不能满足统计学要求,常规方法60 h、改进法50和60 h的3个培养方法收获的细胞数均可满足统计学要求,故后3个方法均取作剂量曲线;②后3个培养方法的PCC环与受照剂量的相关系数其系数间相当接近(均0.996以上、均P<0.01),直线回归方程成立,相关指数亦相当接近,剂量-效应拟合均为线性模式,回归系数间差异无统计学意义(P<0.05),回归直线的形态基本重叠;③验证:本研究的3条剂量-曲线估算受照10.0 Gy的结果误差均≤8%,均符合生物学实验一般允许的15%。结论本研究的3个方法制作的3条曲线均可用于大剂量电离辐射损伤的生物剂量估算,但以改进法的50 h培养速度快,可作为事故应急首选的方法。
Objective To study the effect of routine and modified methods on dose-response curve of 60Coγ-ray in precocious aggregated chromosome (PCC) method and to choose a fast and accurate biological dose estimation method for emergency response. Methods Three elbow vein blood of male aged 23 to 28 years old was collected and cultured under the condition of 60Co γ ray irradiation (01,0.0,5.0,10.0,15.02,0.0 Gy and 0.635 Gy / min absorbed dose) As a comparison. The dose-response curve was compared between the two incubation times of 50 h and 60 h of the two methods (conventional and modified). Another laboratory was irradiated with a dose-response curve of 10.0 Gy (0.670 Gy / min ) The resulting PCC loop results were validated using the dose-curve estimated dose produced above. Results ① The number of PCC cells obtained at 15.0 and 20.0 Gy in conventional 50 h culture method did not meet the statistical requirements. The number of cells harvested by conventional culture method at 60 h and 50 h and 60 h improved the statistical requirements, (P> 0.05). The linear regression equation was established, and the correlation index (P <0.01) was obtained between the three methods. (P <0.05), and the regression line basically overlapped. ③ Validation: The three dose-curve estimates in this study were calculated according to the data of 10.0 Gy results were ≤ 8% error, are in line with biological experiments generally allow 15%. Conclusions All three curves produced by the three methods in this study can be used to estimate the biological dose of high dose ionizing radiation injury. However, the 50 h culture method can be used as the first choice for accident emergency.