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目的:了解和揭示滋阴活血润肠法对SD大鼠烫伤后一周内肠壁组织T淋巴细胞和IgA浆细胞数量的变化、血浆肿瘤坏死因子及内毒素的影响,探讨本法对肠黏膜屏障保护作用的机制,以期为临床探寻更有效的肠黏膜保护药物。方法:SD大鼠70只随机分成3组,第一组为滋阴活血润肠中药治疗组(便可通组)30只,第二组为对照组(单纯烫伤组)30只,制备成烫伤模型,分别予以便可通、生理盐水灌胃;第三组为正常组10只;均检测T淋巴细胞和IgA浆细胞数量的变化、血浆肿瘤坏死因子及内毒素的含量。结果:滋阴活血润肠法可明显增加CD3+、CD4+、IgA细胞数量、抑制烫伤模型大鼠内毒素易位,减少肿瘤坏死因子的产生。结论:①中医滋阴活血润肠法有较理想的肠道屏障保护作用,预防肠道屏障功能障碍引发的全身炎性反应综合征和内毒素血症。②滋阴活血润肠法能增加免疫调节功能,是保护肠道屏障的重要机制之一。
Objective: To understand and reveal the effect of nourishing yin and activating blood Runchang method on the changes of T lymphocyte and IgA plasma cell number, plasma tumor necrosis factor (TNF) and endotoxin in intestine wall of SD rats one week after scald, and to explore the effect of this method on intestinal mucosal barrier Protect the mechanism of action, with a view to clinical more effective intestinal mucosal protection drugs. Methods: Seventy SD rats were randomly divided into three groups. The first group consisted of 30 Chinese medicine treatment group (Tongkailong group) with nourishing yin and activating blood and activating intestine, and the other 30 rats in the control group (pure scald group) The rats in the third group were given normal saline. The changes in the number of T lymphocytes and IgA plasma cells and the levels of plasma tumor necrosis factor and endotoxin were detected in the third group. Results: Nourishing Yin and Activating Blood Runchang Method can obviously increase the number of CD3 +, CD4 + and IgA cells, inhibit the endotoxin translocation in rats with scald and reduce the production of tumor necrosis factor. Conclusion: ① TCM Ziyin Huoxue Runchang has a better protective effect on the intestinal barrier to prevent systemic inflammatory response syndrome and endotoxemia caused by intestinal barrier dysfunction. ② Ziyinhuoxue Runchang can increase immune regulation, is one of the important mechanisms to protect the intestinal barrier.