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目的探讨我国汉族人群中8-羟基鸟嘌呤糖苷酶1(hOGG1)基因Ser326Cys多态性与HIV感染间的关系。方法采用病例-对照的流行病学方法,运用PCR-限制性片段多态(restrictionfragment length polymorphism,RFLP)技术,对126例HIV血清学阴性者和154例HIV血清学阳性者hOGG1基因第326位点Ser/Cys多态性进行分析,并比较hOGG1不同基因型与HIV感染间的关系。结果HIV血清学阴性组中3种基因型频率分布与现报道的中国汉族人群结果相近,其Ser/Ser、Ser/Cys和Cys/Cys基因型分布频率分别为20.6%,46.8%和32.5%,HIV血清学阳性组分别为16.2%,51.9%和31.8%,两组间差异无统计学意义(χ2=1.12,P>0.05)。HIV血清学阳性组人群中,合并HCV感染者与未合并HCV感染者Ser/Ser、Ser/Cys和Cys/Cys基因型频率分别为:HCV阳性组20.0%、48.9%和31.1%;HCV阴性组14.7%、53.2%和32.1%,两组间差异无统计学意义(χ2=0.68,P>0.05)。结论本研究未发现hOGG1基因Ser326Cys多态性与HIV感染及HIV与HCV共感染间的相关性。
Objective To investigate the relationship between the serine nucleotide polymorphism of serine 8 - oxoguanosine 1 (hOGG1) gene Ser326Cys and HIV infection in Chinese Han population. METHODS: A case-control epidemiological study was performed on 126 loci of hOGG1 gene in 126 HIV-seronegative and 154 HIV-seropositive women with restriction fragment length polymorphism (PCR-RFLP) Ser / Cys polymorphism were analyzed, and compared hOGG1 different genotypes and HIV infection. Results The frequencies of three genotypes in HIV seronegative group were similar to those reported in Chinese Han population. The frequencies of Ser / Ser, Ser / Cys and Cys / Cys genotypes were 20.6%, 46.8% and 32.5%, respectively. HIV seropositive group were 16.2%, 51.9% and 31.8% respectively, with no significant difference between the two groups (χ2 = 1.12, P> 0.05). Among the HIV seropositive groups, the frequencies of Ser / Ser, Ser / Cys and Cys / Cys genotypes were 20.0%, 48.9% and 31.1% respectively in HCV-positive and HCV-infected individuals with or without HCV infection; HCV-negative 14.7%, 53.2% and 32.1% respectively. There was no significant difference between the two groups (χ2 = 0.68, P> 0.05). Conclusion This study did not find the hOGG1 gene Ser326Cys polymorphism and HIV infection and co-infection between HIV and HCV.