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采用ＤＮＡ浸胚法将瓠瓜ＤＮＡ导入西瓜，在病圃中经３代自交纯化和抗性筛选，在Ｄ３代选育出了Ｄ３－１～Ｄ３－４４份抗性材料。苗期接种鉴定结果表明，Ｄ３－１和Ｄ３－２对西瓜枯萎病高抗，Ｄ３－３和Ｄ３－４对西瓜枯萎病中抗。用感病品种蜜宝作母本，分别以高抗西瓜枯萎病的材料Ｄ３－１和Ｄ３－２作父本杂交，Ｆ１代都表现高抗枯萎病。Ｆ２代及与感病亲本蜜宝回交的ＢＣ１代群体抗、感分离比例都符合３∶１及１∶１的分离比例，说明枯萎病抗性遗传是单基因或单ＤＮＡ片段控制的显性遗传。
DNA was introduced into watermelon by DNA immersion method. After selfing for 3 generations and resistance screening in the nursery, D3-1-D3-44 resistant materials were selected in the D3 generation. Seedling inoculation results showed that D3-1 and D3-2 were highly resistant to watermelon blight and D3-3 and D3-4 were resistant to watermelon blight. With the susceptible variety Mirabilis as female parent, F1-1 and D3-2, which are highly resistant to watermelon blight, were used as the male parent, respectively, F1 generation showed high resistance to Fusarium wilt. The anti-susceptible and susceptible segregation ratios of F2 generation and BC1 population backcrossing with susceptible parents were both in the ratio of 3: 1 and 1: 1, indicating that the resistance to Fusarium wilt was dominated by single-gene or single-DNA fragment control Genetic.