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目的 :研究吸入性糖皮质激素对豚鼠速发性支气管哮喘的快速作用。 方法 :以卵白蛋白为抗原致敏豚鼠 ,抗原再次激发速发性支气管哮喘反应前 ,处理组雾化吸入糖皮质激素布地奈德 (3mg/ml混悬于羟甲基纤维素赋形剂 ) 5min ,对照组雾化吸入赋形剂和生理盐水。实时测量哮喘豚鼠肺功能参数 ,肺动态顺应性 (Cdyn)和肺阻力 (RL)的变化 ,作为反映哮喘严重程度的指标。 结果 :基因组机制介导的糖皮质激素作用需要数小时才能起效 ,而抗原激发前雾化吸入糖皮质激素布地奈德在 10min内明显抑制了豚鼠支气管哮喘反应的Cdyn和RL变化 (P <0 .0 5 )。 结论 :糖皮质激素布地奈德快速抑制豚鼠速发性哮喘 ,为研究糖皮质激素非基因组机制提供了直观的实验基础 ,进一步的研究将可能为糖皮质激素在包括哮喘在内的变态反应性疾病中的临床应用提供理论依据
Objective: To study the rapid effects of inhaled glucocorticoids on guinea pigs with immediate-onset bronchial asthma. Methods: The guinea pigs were sensitized with ovalbumin. Before antigen challenged the patients with immediate asthma, the treatment group was given budesonide (3mg / ml suspension in hydroxymethylcellulose excipient) for 5min , Control group inhalation of nebulizer and saline. Real-time measurement of pulmonary function parameters, changes in lung dynamic compliance (Cdyn) and lung resistance (RL) in asthmatic guinea pigs was used as an index to reflect the severity of asthma. RESULTS: Genomic mechanism-mediated glucocorticoid action took several hours to effect, whereas inhalation of glucocorticoid budesonide prior to antigen challenge significantly inhibited changes in Cdyn and RL in guinea pig bronchial asthma responses within 10 min (P <0 .0 5). CONCLUSION: Budesonide, a glucocorticoid, can rapidly inhibit the onset of guinea pig rapid onset asthma, providing an experimental basis for studying the non-genomic mechanism of glucocorticoid. Further research may be about glucocorticoid in allergic diseases including asthma In the clinical application of theoretical basis