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目的探讨热应激过程中热应激蛋白(HSP)的合成动力学、组织细胞分布及其功能。方法通过体内外实验,采用Westernblot、免疫组化和Northerndotblot等方法。结果小鼠巨噬细胞和人淋巴细胞受热后均见以70kD的HSP合成最多,且与受热强度有关。随着HSP的增多,细胞存活率明显升高,受损伤程度减轻。热应激时受试动物HSP70表达累积量主要在心、肝组织中,且主要位于胞核内,恢复期移出至胞浆。结论HSP70合成水平与分布对热应激后组织细胞保护作用有关
Objective To investigate the synthesis kinetics, tissue cell distribution and function of heat stress protein (HSP) during heat stress. Methods By in vitro and in vivo experiments, using Western blot, immunohistochemistry and Northerndotblot and other methods. Results After heat-induced by mouse macrophages and human lymphocytes, HSPs synthesized by 70kD were the most abundant and correlated with the intensity of heat exposure. With the increase of HSP, cell survival rate was significantly increased, lessened extent of injury. HSP70 accumulation in the test animals was mainly in the heart and liver tissue, mainly in the nucleus, and was removed to the cytoplasm in the recovery period. Conclusion The level of HSP70 synthesis and distribution are related to the protection of tissue cells after heat stress