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[目的]该研究旨在建立大果黑果枸杞组培快繁技术体系。[方法]以大果黑果枸杞嫩茎作为外植体,以MS为基本培养基,研究了不同因素对大果黑果枸杞组培苗初代、继代以及生根培养的影响。[结果]适合大果黑果枸杞初代培养的培养基配方为MS+ZT 0.2 mg/L+IBA0.01 mg/L,在此培养基上,腋芽的生长状况良好,萌芽率高达88.73%,且玻璃化的情况很少;同样,ZT对组培苗增殖培养的效果要优于6-BA,适合组培苗增殖培养的培养基配方为MS+ZT 0.15 mg/L+IBA 0.01 mg/L,在此培养基上,组培苗的生长速度快,其增殖倍数可达5.83倍,组培苗基本没有玻璃化现象,且苗生长健壮;适合组培苗生根的培养基为MS+IBA1.0 mg/L,生根率可达100%。[结论]适合大果黑果枸杞组培苗炼苗移栽的基质配比为腐殖土∶珍珠岩=1∶1,在此基质中培养的苗木均长势良好,成活率高达92.37%。[结论]该研究为大果黑果枸杞的规模化生产及推广提供理论依据。
[Objective] The aim of this study was to establish a tissue culture technique system of Lycium barbarum Maxim. [Method] With the fresh fruit of black fruit of Lycium barbarum as explant and MS as the basic medium, the effects of different factors on the primary, secondary and rooting culture of Lycium barbarum were studied. [Result] The medium for the first culture of Lycium chinense L. was MS + ZT 0.2 mg / L + IBA 0.01 mg / L, and the growth of axillary bud was good on this medium. The germination rate was as high as 88.73% In the same way, the effect of ZT on the proliferation of tissue culture seedlings was better than that of 6-BA. The culture medium suitable for tissue culture was MS + ZT 0.15 mg / L + IBA 0.01 mg / L, In this medium, the growth of tissue culture seedlings fast, the multiplication rate of up to 5.83 times, basically no vitrification of tissue culture and seedlings grow robust; suitable tissue culture rooting medium for the MS + IBA1.0 mg / L, rooting rate up to 100%. [Conclusion] The suitable ratio of matrix for transplanting of Fructus Lycii tissue culture seedlings was humic soil: perlite = 1: 1. All the seedlings cultured in this medium grew well with the survival rate as high as 92.37%. [Conclusion] The study provided the theoretical basis for the large-scale production and popularization of Lycium barbarum fruit.