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目的 探讨一氧化氮 (NOS)与前列腺增生 (BPH)发病的关系以及中药消癃通闭对前列腺平滑肌中NOS神经的影响。 方法 去势雄性大鼠 ,皮下注射丙酸睾酮 5mg/ (kg·d) ,分别给予消癃通闭、保列治灌胃 ,2 1d后断髓处死 ,应用NADPH组化染色结合形态学定量分析方法 ,检测各组前列腺中NOS神经含量。 结果 NOS神经纤维主要分布在前列腺平滑肌细胞周围 ,消癃通闭高剂量组在治疗 3周后 ,前列腺组织中NOS神经的长度密度 (Lv)相对值为 (113.4 9± 2 3.30 )× 10 -3 ,与其它 3组相比均有显著性差异 (P <0 .0 1)。 结论 中药消癃通闭可增加模型BPH大鼠前列腺中NOS神经含量 ,为该药治疗前列腺增生改善临床症状提供了科学依据
Objective To investigate the relationship between nitric oxide (NOS) and the development of benign prostatic hyperplasia (BPH) and the effect of Chinese herbal medicine on the NOS nerves in smooth muscle of prostate. Methods Male ovariectomized rats were injected subcutaneously with testosterone propionate 5 mg/(kg·d). They were given Yi Xiao Tong Tuo and Bao Li Zhi gavage. The rats were sacrificed after 21 days of treatment. NADPH histochemical staining and morphological quantitative analysis were performed. Methods: The content of NOS in the prostate was measured in each group. Results The NOS nerve fibers mainly distributed around the prostate smooth muscle cells. After 3 weeks of treatment, the relative density of NOS nerves in the prostate tissue was (113.4 9±2 3.30) × 10 -3 . Compared with other three groups, there were significant differences (P < 0.01). Conclusions The elimination of phlegm and blood stasis in traditional Chinese medicine can increase the content of NOS in the prostate of model BPH rats and provide a scientific basis for the treatment of benign prostatic hyperplasia and improve the clinical symptoms.