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目的探讨建立一种操作简便、灵敏度和准确度较高的盐硒测定方法。方法称取一定量盐样加水溶解,溶液中硒(Ⅳ)与硼氢化钠反应生成SeH2,然后用氩气载入电热石英炉中原子化,根据产生的荧光强度对溶液中硒进行定量检测。结果运用方法学评价试验得出氢化物原子荧光法测定食盐中的硒,线性范围(0.40~60.00)μg/L,检出限为0.40μg/L,RSD 1.05%,选高、中、低3种浓度的盐样做加标回收率96.81%~105.58%;而紫外分光光度法测硒盐的检出限为50μg/L,回收率92.10%~103.27%,两种方法差异无统计学意义(t0.01)。结论氢化物原子荧光法检出限低、重复性好、准确度高、操作简便、快速,适用于地方病实验室大批量的硒盐检测工作。
Objective To establish a simple, sensitive and accurate method for determination of salt selenium. Methods A certain amount of salt solution was weighed and dissolved in water. Selenium (Ⅳ) reacted with sodium borohydride in the solution to form SeH2, which was then atomized in an electric quartz furnace with argon gas. Quantitative detection of selenium in the solution was performed according to the fluorescence intensity generated. Results The method of hydride generation atomic fluorescence spectrometry was used to determine selenium in salt solution. The linear range (0.40 ~ 60.00) μg / L, the detection limit was 0.40μg / L, RSD 1.05% The recoveries ranged from 96.81% to 105.58%. The UV detection of selenium was 50μg / L and the recoveries were 92.10% ~ 103.27%. There was no significant difference between the two methods t 0.01). Conclusions Hydridexofluorescence method has the advantages of low detection limit, good repeatability, high accuracy, simple and rapid operation, and is suitable for large quantities of selenium salt detection in endemic disease laboratories.