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目的探讨P16及CyclinD1在小儿急性白血病中的表达。方法采用免疫组化S-P法检测39例小儿急性白血病及10例正常对照者骨髓中P16与CyclinD1蛋白的表达,并结合临床病理资料进行分析。结果P16及CyclinD1蛋白在小儿急性白血病患者表达率分别为31%和54%,在对照组表达率为70%和0。两者在白血病与对照组中的表达差异有统计学意义。在37例接受化疗的儿童急性白血病中,P16阳性表达组化疗后的缓解率(66%)明显高于P16表达阴性组的缓解率(32%),差异有统计学意义(P<0.05)。P16与CyclinD1在白血病中的表达具有正相关性。结论P16与CyclinD1在小儿急性白血病患者存在异常表达,其蛋白的表达水平可能会影响化疗疗效。
Objective To investigate the expression of P16 and CyclinD1 in children with acute leukemia. Methods Immunohistochemical S-P method was used to detect the expression of P16 and CyclinD1 in 39 pediatric acute leukemia and 10 normal controls, and analyzed with clinicopathological data. Results The positive rates of P16 and CyclinD1 in children with acute leukemia were 31% and 54%, respectively, and those in control group were 70% and 0% respectively. There was significant difference between the two in leukemia and control group. In 37 children with acute leukemia treated with chemotherapy, the response rate of P16-positive group was significantly higher than that of P16-negative group (66%) (P <0.05). P16 and CyclinD1 expression in leukemia has a positive correlation. Conclusions P16 and CyclinD1 are abnormally expressed in children with acute leukemia and their protein expression may affect the efficacy of chemotherapy.