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用Ｎｏｒｔｈｅｒｎ印迹杂交和放射性配基结合法，检测了百日咳杆菌脑损伤时大鼠大脑皮质ＮＭ－ＤＡ受体的变化。结果显示：注菌组Ｋｄ值较对照组小（Ｐ＜０．０５），ＮＭＤＡＲ１ｍＲＮＡ表达及Ｂｍａｘ值较对照组差异无显著性（Ｐ＞０．０５）。提示ＮＭＤＡ受体主要表现为受体功能的激活，亲和力增加，并非基因表达的改变而使受体的数量变化。
Northern blot hybridization and radioligand binding assay were used to detect the changes of NM-DA receptor in rat cerebral cortex during Bordetella pertussis brain injury. The results showed that the Kd value of the bacterium group was lower than that of the control group (P <0.05), and the expression of NMDAR1 mRNA and the Bmax value were not significantly different from the control group (P> 0.05). It is suggested that the NMDA receptor mainly displays the activation of receptor function and the increase of affinity. It is not the change of gene expression that changes the number of receptors.