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对发酵液中纳他霉素的色谱分离条件进行了优化,分别探讨了流动相的组成、冰醋酸水溶液与甲醇的比例、流动相中冰醋酸浓度对保留时间的影响,确定了纳他霉素的分离条件。该方法在5.0 mg/L～300 mg/L范围内呈良好的线性关系,相关系数r~2=0.999 8。纳他霉素的加标回收率在100.07%～102.41%之间。相对标准偏差(KSD)小于1.56%(n=3)。最低检测限为0.25μg/mL(S/N=3)。用甲醇超声提取样品,并通过优化的色谱条件对其进行分析,测定了发酵液中纳他霉素的含量。
The conditions for the chromatographic separation of natamycin in the fermentation broth were optimized. The effects of the mobile phase composition, the ratio of glacial acetic acid to methanol and the concentration of glacial acetic acid in the mobile phase on the retention time were investigated respectively. The effects of natamycin The separation conditions. The method showed a good linearity in the range of 5.0 mg / L to 300 mg / L with a correlation coefficient of r = 2 = 0.999 8. The natamycin spiked recoveries ranged from 100.07% to 102.41%. The relative standard deviation (KSD) was less than 1.56% (n = 3). The lowest limit of detection was 0.25 μg / mL (S / N = 3). Samples were extracted with methanol and analyzed by optimized chromatographic conditions. The content of natamycin in the fermentation broth was determined.