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恶性疟原虫对二氢叶酸还原酶抑制剂和氯喹的抗性,已被定位于单一的基因位点。二氢叶酸还原酶基因的特定点突变造成了对两种二氢叶酸抑制剂即环氯胍和乙胺嘧啶的抗性,抗性程度随点突变的位置和所涉及的氨基酸残基的不同而有所差异。氯喹抗性位点已被定位于恶性疟原虫杂交株第7号染色体中的一个400kb的片段之上。阐明该片段内基因的特性将有助于理解引起氯喹抗性的药物快速外流机制。
Plasmodium falciparum resistance to dihydrofolate reductase inhibitors and chloroquine has been mapped to a single locus. Specific point mutations in the dihydrofolate reductase gene result in resistance to two dihydrofolate inhibitors, cycloguanil and pyrimethamine, with varying degrees of resistance depending on the location of the point mutation and the amino acid residues involved Differences. The chloroquine resistance site has been mapped to a 400 kb fragment in chromosome 7 of the P. falciparum hybrid. The elucidation of the characteristics of the gene in this fragment will help to understand the mechanism of rapid drug efflux that causes chloroquine resistance.