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实验目的:研究18个短串联重复序列(Short Tandem Repeat,STR)位点(D5S818、D21S11、D7S820、CSF1PO、D2S1338、D3S1358、vWA、D8S1179、D16S539、Penta E、TPOX、TH01、D19S433、D18S51、FGA、D6S1043、D13S317、D12S391)在新疆克拉玛依地区人群中的基因频率分布。实验方法:采用PCR扩增及毛细管电泳技术对332名个体的18个STR基因座进行分析。实验结果:共检出213种等位基因,基因频率分布在0.002~0.536之间。18个STR基因型分布均符合Hardy-Weinberg平衡(P>0.05),杂合度均不低于0.589,个人识别能力均不小于0.795,多态信息含量均不小于0.56,非父排除概率均不小于0.278。结论:本文研究了新疆克拉玛依地区人群18个STR位点的遗传多态性,为人类群体遗传学及法医学后续研究提供详实可靠的基础数据。
The aim of this study was to investigate the effect of 18 short Tandem Repeat (STR) sites (D5S818, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, vWA, D8S1179, D16S539, Penta E, TPOX, TH01, D19S433, D18S51, FGA , D6S1043, D13S317, D12S391) in the population of Karamay region in Xinjiang. Experimental Methods: Eighteen STR loci in 332 individuals were analyzed by PCR amplification and capillary electrophoresis. Experimental results: A total of 213 kinds of alleles were detected, the gene frequency distribution between 0.002 ~ 0.536. The distributions of 18 STR genotypes were in accordance with Hardy-Weinberg equilibrium (P> 0.05), the heterozygosity was no less than 0.589, the individual recognition ability was no less than 0.795, the polymorphism information content was no less than 0.56, and the non-parent exclusion probability was not less than 0.278. CONCLUSION: This study investigated the genetic polymorphism of 18 STR loci in Karamay, Xinjiang, and provided detailed and reliable basic data for the follow-up study of human population genetics and forensic science.