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凝集素是一类能与糖特异结合的蛋白质,当与细胞表面的糖结合时,由于含多个结合部位,可交联大量细胞引起凝集。凝集素与糖的结合有选择性,因此可以用以研究细胞表面糖谱,是研究细胞结构很好的探针,尤其是凝集素能凝集恶变细胞,这对肿瘤的研究开辟了新领域。利用凝集一方面可与酶或生物素标记物结合,另一方面又可与细胞膜上糖基结合的亲和组织化学特征,就有可能应用免疫组织学技术来研究细胞分化,增生和恶变的生物学过程。胃肠道粘液是糖蛋白,不同的粘液蛋白有不同的糖基侧链,我们应用卵白素(Avidin)、生物素(Biotin)—PHA、PNA、CONA,以及生
Lectins are proteins that specifically bind to sugars. When combined with sugars on the cell surface, they can crosslink a large number of cells and cause aggregation due to multiple binding sites. The binding of lectin to sugar is selective, so it can be used to study the cell surface glycometabolism. It is a good probe for studying cell structure, especially lectin can agglutinate malignant cells, which opens up a new field for the study of tumors. The use of agglutination, on the one hand, in combination with an enzyme or a biotin label, and on the other hand, with the affinity of the glycosyl groups on the cell membrane, makes it possible to apply immunohistological techniques to study cell differentiation, proliferation and malignant organisms. Learning process. Gastrointestinal mucus is a glycoprotein. Different mucin proteins have different sugar side chains. We use Avidin, Biotin-PHA, PNA, CONA, and Raw.