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在稀HCl介质中,I_3~-在340 nm处有一吸收峰;当小檗碱(BB)与I_3~-共存时体系呈橙黄色,在580 nm处产生一共振散射峰。以试剂作参比,该缔舍微粒体系在530 nm产生一吸收峰,BB浓度在0～7.0×10~(-5)mol/L范围内与A_(530nm)呈线性,据此建立了一种测定小檗碱含量的分光光度新方法,并用于针剂样品中小檗碱测定,结果满意。同步散射光谱研究表明,BB~+与I_3~-可通过静电引力作用形成疏水性的(I_3-BB)缔合物分子,并进一步聚集形成稳定的(I_3-BB)_n缔合纳米微粒。由于该缔合纳米微粒在580 nm处产生共振散射效应,故体系呈橙黄色。
In dilute HCl medium, I_3 ~ - has an absorption peak at 340 nm. When berberine (BB) and I_3 ~ - coexist, the system shows an orange-yellow color and produces a resonant scattering peak at 580 nm. Using reagent as a reference, the detached microparticle system produced an absorption peak at 530 nm. The concentration of BB was linear with A 530 nm in the range of 0-7.0 × 10 -5 mol / L, A New Method for Spectrophotometric Determination of Berberine Content and Its Application in Determination of Berberine in Injection Samples with Satisfactory Results. The results of synchrotron scattering show that BB ~ + and I_3 ~ - can form hydrophobic (I_3-BB) associate molecules by electrostatic attraction and further aggregate to form stable (I_3-BB) _n association nanoparticles. Since the association nanoparticles produce resonance scattering effect at 580 nm, the system is orange-yellow.