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目的:建立以高效液相色谱法测定护肝宁片中大黄素含量的方法。方法:色谱柱为C18,流动相为甲醇—0.2%磷酸溶液(85:15),流速为1ml/min,检测波长为254nm,柱温为室温。结果:大黄素检测浓度在20μg/ml-200μg/ml范围内线性关系良好(r=0.9999),平均加样回收率为98.63%(RSD为1.19%)。结论:本方法操作简便、精密度好、结果可靠,可用于护肝宁片的质量控制。
Objective: To establish a HPLC method for the determination of emodin in Huganing Tablets. Methods: The chromatographic column was C18, the mobile phase was methanol-0.2% phosphoric acid solution (85:15), the flow rate was 1 ml/min, the detection wavelength was 254 nm, and the column temperature was room temperature. RESULTS: The calibration curve showed a good linear relationship (r=0.9999) in the range of 20μg/ml-200μg/ml, and the average recovery was 98.63% (RSD was 1.19%). Conclusion: This method is simple, accurate, reliable, and can be used to control the quality of Huganing tablets.