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本文以H_2O_2诱导损伤的大鼠肾上腺嗜铬细胞瘤PC12细胞为细胞模型,分别研究了三种蛋白源酶解产物(脑活素、鳕鱼蛋白肽和核桃蛋白肽)对H_2O_2诱导PC12细胞氧化损伤的保护作用。结果表明,H_2O_2的加入会使得PC12细胞的细胞数目减少,部分胞体皱缩成圆形或椭圆形,周围光晕消失,且随着H_2O_2浓度的增加,细胞存活率逐渐降低。脑活素可获得最好的PC12细胞损伤保护作用,核桃蛋白肽次之,而鳕鱼蛋白肽作用不明显;此外,结果表明脑活素、鳕鱼蛋白肽和核桃蛋白肽对受损伤细胞的存活和增殖也能起一定的改善作用。因此,核桃蛋白肽是一种改善脑部功能性障碍的潜在原料。
In this paper, adrenal pheochromocytoma PC12 cells induced by H 2 O 2 were used as the cell model. The effects of three proteolytic products (cerebrolysin, cod protein peptide and walnut peptide) on oxidative damage of PC12 cells induced by H 2 O 2 were studied respectively Protective effects. The results showed that the addition of H 2 O 2 decreased the number of cells in PC12 cells, and some cells collapsed into a circle or ellipse. The surrounding halo disappeared. With the increase of H 2 O 2 concentration, the cell viability decreased gradually. Cerebrolysin can obtain the best protective effect of PC12 cell injury, followed by walnut protein peptide, while the effect of cod-fish protein peptide is not obvious. In addition, the results indicate that survivin of damaged cells and the activity of cerebrolysin, cod-peptide and walnut- Proliferation can also play a role in improving. Therefore, walnut protein peptide is a potential raw material to improve brain functional disorders.