论文部分内容阅读
目的建立快速、简易的检测鼠疫F1抗体的胶体金免疫层析法(G ICA)。方法(1)采用胶体金颗粒标记纯化F1抗原,并将标记物喷于玻璃纤维;同时将纯化F1抗原喷线固定于硝酸纤维素膜上,用于F1抗体的捕捉;按常规组装成检测鼠疫抗体免疫层析试纸条。(2)采用该试纸条与血凝法对同一份兔抗F1抗体进行检测,以评价该试纸条的敏感性。(3)采用该试纸条对44株非鼠疫菌的免疫鼠血清进行检测,以评价该试纸条的特异性。(4)采用该试纸条、血凝法及ELISA对607份血清标本进行检测,以评价该试纸条对现场材料的检测效果。结果(1)该试纸条可在15 m in之内完成检测;(2)在敏感性上,该试纸条对同一份免疫兔血清的检测较血凝法高一个滴度;(3)对被试的44株所选菌株的免疫鼠血清的检测均为阴性;(4)在对607份血清标本的检测中,免疫层析试纸条、血凝及ELISA三种方法的符合率中度,而免疫层析试纸条的敏感性分别比血凝与ELISA高111%和90%。结论以纯化的鼠疫F1抗原为基础建立的G ICA检测鼠疫F1抗体的方法特异性强、灵敏度高、简便快速,无需特殊仪器设备,有较大的推广应用价值。
Objective To establish a rapid and simple colony gold immunochromatographic assay (G ICA) for the detection of F1 antibody in plague. Methods (1) Purified F1 antigen with colloidal gold particles and sprayed onto glass fiber; immobilized purified F1 antigen on nitrocellulose membrane for capture of F1 antibody; routinely assembled to detect plague Antibody immunochromatographic strip. (2) using the test strips and hemagglutination method to test the same rabbit anti-F1 antibody to evaluate the sensitivity of the test strip. (3) The test strip was used to test 44 non-Yersinia pestis-immunized rat sera to evaluate the specificity of the test strip. (4) The test strips, hemagglutination and ELISA were used to test 607 serum samples to evaluate the test results of the test strips on the field materials. Results (1) The test strip can be tested within 15 mins; (2) The sensitivity of the test strip to the same immunized rabbit serum is one titer higher than that of the coagulation method; (3) (4) In the detection of 607 serum samples, the coincidence rate of three methods of immunochromatographic test strip, hemagglutination and ELISA Degrees, while the immunochromatographic strips were 111% and 90% more sensitive than hemagglutination and ELISA, respectively. Conclusion The G ICA method based on the purified plague F1 antigen has the advantages of high specificity, high sensitivity, simple and rapid detection without any special instruments and equipment, which is of great value in popularization and application.