以青州蜜桃、冬桃成熟胚子叶为外植体 ,研究了利用子叶再生不定梢的适宜培养基。两个品种的子叶在 1 / 2MS附加BA 1 0mg/L和NAA 0 .2mg/L的培养基上获得了最高不定梢再生率。青州蜜桃和冬桃子叶的不定梢最高再生率分别是 6 6 .7%和 5 6 .2 %。不定梢在WPM +BA 2mg/L +IBA 0 .0 1mg/L +GA 0 .5mg/L培养基上增殖培养后 ,转移到 1 / 2MS +IBA 0 .5mg/L +蔗糖 2 0g/L诱导生根 ,两个品种的生根率都在 90 %以上 Taking Qingzhou peach, winter mature embryo leaves as explants, suitable medium for adventitious shoot regeneration using cotyledons was studied. Cotyledons of both cultivars achieved the highest shoot regeneration rate on medium supplemented with BA 1 0 mg / L and NAA 0.2 mg / L. The highest adventitious shoot regeneration rates of Qingzhou peach and winter peach leaves were 66.7% and 56.2%, respectively. The adventitious shoots were induced to proliferate on the medium of WPM + BA 2mg / L + IBA0.1mg / L + GA0.5mg / L and then transferred to 1/2 MS + IBA0.5mg / L + sucrose20g / L Rooting, the rooting rate of the two varieties are more than 90%