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取牛心肌加无离子水于+4℃磨成匀浆,按Kirby的苯酚法分离RNA,先用100g离心45分钟,然后用10,000g离心20分钟。每100克牛心肌可得30～50毫克RNA,用甲基苯二酚法测RNA含量。RNA中各种微量元素用中子活化法
Bovine myocardium plus deionized water was ground to homogeneity at + 4 ° C. RNA was isolated by Kirby’s phenol method. Centrifuge at 100g for 45 minutes and then 10,000g for 20 minutes. 30 to 50 milligrams of RNA per 100 grams of bovine myocardium can be measured using the methylhydroquinone RNA method. Various trace elements in RNA using neutron activation method