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目的:观察比较iNOS抑制药AG对鼠离体主动脉和肺动脉环张力的影响。方法:测定血管对NE的量-效反应,在LPS(300ng/ml)中孵育4小时后。分为L-NAME(300μmol/L)或AG(100μmol/L)孵育60或20分钟共8组。结果:LPS孵育后动脉环对NE的反应明显降低,给予L-NAME或AG后均升高以10~(-6)mol/L NE作比较,L-NAME组主动脉20分钟时反应性明显高于AG组。L-NAME组肺动脉较AG组明显升高(P<0.01)。AG60分钟组主动脉升高的幅度明显大于肺动咏组(P<0.01)。L-NAME引起的血管收缩高峰时间较AG组出现的早,且峰值高。结论:L-NAME和AG部可增加已降低的对NE的反应性;但AG仅使主动脉环的敏感性明显增加。
Objective: To observe and compare the effect of iNOS inhibitor AG on the tension of isolated aorta and pulmonary artery in vitro. Methods: The amount-response of blood vessels to NE was measured and incubated for 4 hours in LPS (300 ng / ml). Divided into L-NAME (300μmol / L) or AG (100μmol / L) for 60 or 20 minutes and a total of 8 groups. Results: After LPS incubation, the response of the arterial rings to NE was significantly reduced. After L-NAME or AG administration, both were increased with 10 -6 mol / L NE. The aorta in L-NAME group showed a significant response at 20 min Higher than the AG group. The pulmonary artery in L-NAME group was significantly higher than that in AG group (P <0.01). The amplitude of aortic elevation in AG60 minutes group was significantly larger than that in pulmonary artery group (P <0.01). L-NAME induced vasoconstriction peak earlier than the AG group, and the peak was high. CONCLUSIONS: L-NAME and AG increased the decreased response to NE; however, AG increased the sensitivity of the aortic annulus only significantly.