目的 :探讨分泌性中耳炎的发病原因 ,为临床治疗提供理论依据。 方法 :通过微波烧灼双侧咽鼓管口 ,建立分泌性中耳炎动物模型。光镜观察比较咽鼓管管腔及黏 软骨膜和鼓室黏膜变化。电镜观察咽鼓管鼓室段暗颗粒分泌细胞及其表面活性物质样板层体的变化。结果 :各组咽鼓管管腔通畅 ,咽鼓管黏 软骨膜不易受炎症浸润及负压影响。鼓室黏膜水肿、增生、炎性细胞浸润明显 ,暗颗粒细胞分泌功能受损 ,表面活性物质板层体结构明显减少或消失。结论 :咽鼓管黏 软骨膜在分泌性中耳炎发病过程中起重要作用。表面活性物质减少与分泌性中耳炎的发生密切相关 Objective: To investigate the pathogenesis of secretory otitis media, to provide a theoretical basis for clinical treatment. Methods: The bilateral eustachian tube orifice was cauterized by microwave, and the animal model of secretory otitis media was established. Light microscope observation and comparison of Eustachian tube mucosa and mucosa and tympanic mucosa changes. Electron microscopy observation of eustachian tube tympanosclerosis secretory cells and its surface active substance layer changes. Results: The eustachian tube lumen of each group was smooth. Eustachian mucoperiosteum was not easily affected by inflammatory infiltration and negative pressure. Tympanic mucosa edema, hyperplasia, inflammatory cell infiltration, impaired secretory function of dark granular cells, surface layer structure of the material significantly reduced or disappeared. Conclusion: Eustachian tube perichondrium plays an important role in the pathogenesis of secretory otitis media. The reduction of surfactant is closely related to the occurrence of secretory otitis media