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目的探讨致癫痫持续状态幼鼠(status epilepticus,SE)成年后发展为自发反复性癫痫发作(spontaneous recurrent epileptic seizure,SRS)的大鼠脑内髓鞘的变化。方法采用氯化锂-匹罗卡品腹腔注射出生后20～28d体质量(70±5)g雄性SD幼鼠SE模型,应用多道生理信号采集处理系统检测大鼠脑电图(electroencephalogram,EEG)异常活动波,采用Nissl染色方法观察脑内神经元的变化,证实SRS模型大鼠诱导成功;应用卢卡斯快蓝(luxol fast blue,LFB)染色及髓鞘碱性蛋白(myelin basic protein,MBP)免疫组织化学染色等技术观察脑内髓鞘的变化。结果 SRS模型组脑电图与正常对照组相比,可见频发尖波、棘-慢、尖-慢综合波;Nissl染色显示神经元数目在海马CA1(242.1±33.6)、CA3(354.8±26.8)和齿状回门区(61.8±8.3)减少(P<0.01),部分细胞变性和坏死。LFB染色可见SRS模型组在胼胝体(callosumcorpus,cc;0.162±0.017)、扣带回(cingulated gyrus,cg;0.139±0.013)与海马白质(0.080±0.007)区域内的着色范围明显缩小,着色浅淡,与正常对照组相比有显著差异(P<0.01);MBP免疫阳性沉淀在cc(0.183±0.013)、cg(0.192±0.016)、第一躯体感觉皮质区(S1BF;0.076±0.012)和第一听皮质区(Au1;0.085±0.014)内的平均光密度值(OD)显著低于正常对照组(P<0.01)。结论致痫幼鼠成年后伴有慢性癫痫发作可造成中枢神经系统髓鞘脱失。
Objective To investigate the changes of myelin in the brain of rats with status epilepticus (SE) after spontaneous recurrent epileptic seizure (SRS). Methods Seventy-five male Sprague-Dawley (SD) pups aged 20-28 days were injected intraperitoneally with lithium-pilocar injection. The SE model of electroencephalogram (EEG) ) Abnormal activity wave, Nissl staining was used to observe the changes of intracerebral neurons, and it was confirmed that SRS model rats were successfully induced. Using luxol fast blue (LFB) staining and myelin basic protein MBP) immunohistochemical staining and other techniques to observe changes in the brain myelin. Results The Nissl staining showed that the number of neurons in hippocampal CA1 (242.1 ± 33.6) and CA3 (354.8 ± 26.8) was significantly higher in the SRS model group than in the normal control group ) And dentate gyrus (61.8 ± 8.3) (P <0.01), some of the cells degeneration and necrosis. LFB staining showed that the staining range of the SRS model group was significantly reduced in the areas of callosum corpus (cc; 0.162 ± 0.017), cingulated gyrus (cg; 0.139 ± 0.013) and hippocampal white matter (0.080 ± 0.007) (P <0.01). The immunopositive MBP immunoprecipitations showed significant differences between the two groups (cc (0.183 ± 0.013), cg (0.192 ± 0.016), the first somatosensory cortex (S1BF; 0.076 ± 0.012) The average optical density (OD) in the cortical area (Au1; 0.085 ± 0.014) was significantly lower than that in the normal control group (P <0.01). Conclusion Epileptic infant adult rats with chronic seizures can cause demyelination of the central nervous system.