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目的：研究电鳐乙酰胆碱酯酶（ＡＣｈＥ）结构功能关系，探讨ＡＣｈＥ负矩部位是否是其构象决定簇的一个组成部分．方法：用ＥＬＩＳＡ及酶抑制实验观察ＡＣｈＥ负矩部位探针２ＰＡＭ对ＡＣｈＥ与其ＭｃＡｂ３Ｆ３之间的免疫反应性的影响．结果：ＭｃＡｂ３Ｆ３不能与２ＰＡＭ及ＡＣｈＥ的复合物反应；２ＰＡＭ浓度依赖性地降低ＭｃＡｂ３Ｆ３对ＡＣｈＥ的抑制率；但不能解离ＡＣｈＥ与３Ｆ３构成的抗原抗体复合物．结论：电鳐ＡＣｈＥ活性中心负矩部位构成其活性中心构象抗原决定簇的一部分．
Objective: To investigate the functional relationship of acetylcholinesterase (AChE) structure and to explore whether AChE negative-moment site is an integral part of its conformational determinants. Methods: ELISA and enzyme inhibition experiments AChE negative moment probe 2 PAM on AChE and its McAb3F3 immunoreactivity between. Results: McAb3F3 could not react with 2PAM and AChE complexes; 2PAM decreased the inhibitory rate of McAb3F3 to AChE in a concentration-dependent manner; however, it could not dissociate the antigen-antibody complex formed by AChE and 3F3. CONCLUSION: The negative-moment site of AChE activity center in Euphemism constitutes part of the conformational epitope of active center.