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目的利用大孔吸附树脂探索毛菊苣降糖有效部位的最佳纯化工艺。方法以静态吸附法考查9种大孔树脂(HPD100、HPD300、HPD450、HPD600、HPD750、X-5、ADS-17、D4020、D140)对有效部位的吸附和解吸附性能,结合纯化后有效部位对糖尿病及其并发症相关的蛋白酪氨酸磷酸酶1B(PTP-1B)、醛糖还原酶(AR)的抑制率及ABTS抗氧化能力的评价,通过综合评分选出纯化效果最佳的树脂进行进一步研究,确定出最佳纯化工艺。结果确定HPD100大孔树脂为最佳纯化树脂;最佳上样药液质量浓度为75 mg/mL;上样量6 BV;吸附速率为3 BV/h。洗脱前先用水洗(5 BV/h)4 BV除杂,再用60%乙醇以3 BV/h速度洗脱5 BV获得有效部位。结论经HPD100大孔树脂处理后的毛菊苣降糖有效部位对PTP-1B抑制活性可达到纯化前的10倍。该方法简单易行,对提高活性有明显效果,并对工业化生产有指导意义。
Objective To explore the optimum purification process of the effective hypoglycemic sites of Mao chicory with macroporous adsorption resin. Methods The adsorption and desorption properties of nine kinds of macroporous resins (HPD100, HPD300, HPD450, HPD600, HPD750, X-5, ADS-17, D4020 and D140) were studied by static adsorption method. And its complication-related protein tyrosine phosphatase 1B (PTP-1B), aldose reductase (AR) inhibition rate and ABTS anti-oxidation ability, through the comprehensive score selected the best purification of the resin to further Study to determine the best purification process. The results showed that HPD100 macroporous resin was the best resin for purification. The optimum loading liquid mass concentration was 75 mg / mL; the loading volume was 6 BV; the adsorption rate was 3 BV / h. Elution before washing (5 BV / h) 4 BV impurity, then 60% ethanol at 3 BV / h speed of 5 BV to obtain the effective site. Conclusion HPD100 macroporous resin treatment of Mao chicory hypoglycemic effective site of PTP-1B inhibitory activity up to 10 times before purification. The method is simple and easy to operate, has obvious effect on improving the activity and has guiding significance for industrialized production.