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采用小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验、鼠伤寒沙门氏菌营养缺陷型回复突变试验、人外周血淋巴细胞姐妹染色单体交换试验、人外周血淋巴细胞微核试验、中国仓鼠卵巢细胞染色体畸变试验,对人参、绞股蓝、黄芪、甘草和女贞子水提物的低、中、高剂量抗诱变性能进行了实验对比研究.结果显示,人参、绞股蓝、黄芪的中、高剂量组,甘草3个剂量组均能降低环磷酰胺(CP)诱发的微核率(p<0.05);绞股蓝、甘草3个剂量组,人参高剂量组,黄芪中、高剂量组对CP诱发的染色体畸变率增高有抑制效应(p<0.05);人参、绞股蓝、甘草3个剂量组,黄芪中、高剂量组均能抑制由2,7-AF诱发的TA98菌株回变菌落数增加(p<0.05);黄芪、女贞子3个剂量组,绞股蓝中、高剂量组均能抑制由NaN3诱发的TA100菌株回变菌落数增高(p<0.05);绞股蓝中、高剂量组,甘草中剂量组能降低由MMC诱发的SCE增高(p<0.05);人参中、高剂量组,绞股蓝、甘草3个剂量组、女贞子低、中剂量组对MMC诱发的MN增高有抑制作用(p<0.05);人参中、高剂量组,绞股蓝、甘草3个剂量组,女贞子低、中剂量组对MMC诱发的染色体畸变有抑制作用(p<0.05).表明绞股蓝、甘草、人参的抗诱变性能良好,作用范围较广;黄芪、女贞子具有一定的抗诱变作用,但有局限性.
Micronucleus test of bone marrow polychromatic erythrocytes in mice, chromosome aberration test in mouse bone marrow cells, auxotrophic back mutation test in Salmonella typhimurium, sister chromatid exchange test in human peripheral blood lymphocytes, and micronucleus test in human peripheral blood lymphocytes In the chromosomal aberration test of Chinese hamster ovary cells, the low-, medium-, and high-dose antimutagenic properties of ginseng, gynostemma pentaphyllum, astragalus, licorice and Ligustrum lucidum were compared experimentally. The results showed that ginseng, gynostemma pentaphyllum, and jaundice The medium and high dose groups and the three doses of Glycyrrhiza uralensis can reduce the rate of micronucleus induced by cyclophosphamide (CP) (p<0.05); Gynostemma pentaphyllum and licorice three dose groups, high dose group of ginseng, middle and high dose group of astragalus There was an inhibitory effect on the increase of chromosomal aberration induced by CP (p<0.05); Ginseng, Gynostemma pentaphyllum and Glycyrrhiza in three dose groups, medium and high doses of Astragalus membranaceus could inhibit the number of colonies that were reverted by 2,7-AF-induced strain TA98. Increased (p<0.05); Astragalus membranaceus, Ligustrum lucidum three dose groups, gynostemma medium and high dose group can inhibit the NaN3-induced strains of TA100 strain retrogradation increased (p <0.05); Gynostemma medium and high dose group, Licorice Medium Dose It can reduce the increase of SCE induced by MMC (p<0.05); Ginseng middle and high dose group, Gynostemma pentaphylla and Glycyrrhizae three dose groups, Ligustrum lucidum low and middle dose group can inhibit the increase of MN induced by MMC (p<0.05 Ginseng middle and high dose group, Gynostemma pentaphyllum and licorice three dose groups, Ligustrum lucidum low and middle dose group had inhibitory effect on chromosome aberration induced by MMC (p<0.05). It showed that the anti-mutagenic effect of Gynostemma pentaphyllum, Glycyrrhiza uralensis and Ginseng. Good performance, a wide range of effects; Astragalus, Ligustrum lucidum has a certain anti-mutagenic effect, but there are limitations.