[目的]探讨缺氧诱导因子-1α(HIF-1α)、骨桥蛋白(OPN)及c-Met在胆管癌组织中的表达及其临床意义。[方法]采用免疫组化SP法检测HIF-1α、OPN及c-Met在56例胆管癌、30例癌旁正常胆管组织中的表达情况,并分析其与临床病理特征关系及三者的相关性。[结果 ]HIF-1α、OPN及c-Met在胆管癌组织中的表达均明显高于癌旁正常胆管组织,并且在胆管癌组织中表达与患者年龄、性别、肿瘤大小、肿瘤部位、分化程度无相关性(P>0.05),而与肿瘤分期、有无淋巴结转移有关(P<0.05)。HIF-1α、OPN及c-Met在胆管癌组织样本中表达均呈正相关(P<0.05)。[结论]HIF-1α、OPN及c-Met在胆管癌组织中高表达,与肿瘤侵袭转移关系密切。 [Objective] To investigate the expression and clinical significance of hypoxia inducible factor-1α (HIF-1α), osteopontin (OPN) and c-Met in cholangiocarcinoma. [Method] The expressions of HIF-1α, OPN and c-Met in 56 cholangiocarcinoma and 30 paracancerous normal bile duct tissues were detected by immunohistochemical SP method. The relationship between the expression of HIF-1α, OPN and c- Sex. [Results] The expressions of HIF-1α, OPN and c-Met in cholangiocarcinoma tissues were significantly higher than those in normal paracancer tissues, and were significantly correlated with age, gender, tumor size, tumor location, differentiation No correlation (P> 0.05), but with the tumor stage, with or without lymph node metastasis (P <0.05). The expressions of HIF-1α, OPN and c-Met in cholangiocarcinoma tissues were positively correlated (P <0.05). [Conclusion] HIF-1α, OPN and c-Met are overexpressed in cholangiocarcinoma tissue and have a close relationship with tumor invasion and metastasis.