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目的:整合表征银杏黄酮组分整体在水中及不同pH的磷酸盐缓冲液(PBS)中平衡溶解度。方法:采用HPLC同时测定银杏叶提取物中槲皮素、山柰酚、异鼠李素3种黄酮类成分的含量和3类成分在水和不同pH缓冲溶液中的平衡溶解度,进一步通过质量分数权重系数整合法,整合表征银杏黄酮组分的平衡溶解度和油水分配系数。结果:银杏黄酮组分水溶性良好,在pH 7.8时平衡溶解度达到最大,即408.29 mg.L-1。可以初步预测银杏黄酮组分有较好的应用价值,为其组分的制剂成型提供一定的指导依据。结论:比较直接加和法和质量分数权重系数整合法表征银杏黄酮组分性质值,发现质量分数权重系数整合表征中药组分性质更为科学合理,通过该尝试性试验可以为中药组分的性质表征提供一定的研究思路。
OBJECTIVE: To systematically characterize the equilibrium solubility of ginkgo flavone components as a whole in phosphate buffered saline (PBS) in water and at different pHs. Methods: Simultaneous determination of three flavonoid constituents of quercetin, kaempferol and isorhamnetin in Ginkgo biloba extract and the equilibrium solubility of three components in water and different pH buffer solutions were determined by HPLC. Weight coefficient integration method, the integration of the components of the equilibrium solubility of ginkgo flavonoids and oil-water partition coefficient. Results: The components of Ginkgo flavone had good water-solubility, and the equilibrium solubility reached the maximum at pH 7.8 (408.29 mg.L-1). It can preliminarily predict the application value of flavonoids from Ginkgo biloba and provide some guidance for the preparation of its components. Conclusion: Compared with the direct addition method and the mass fraction weight coefficient integration method to characterize the components of ginkgo flavonoids, it is found that the mass fraction weight coefficient is more scientific and reasonable to integrate and characterize the components of traditional Chinese medicine. Through this experimental test, Characterization provides some research ideas.