论文部分内容阅读
目的对645例HIV抗体筛查阳性标本确证实验结果进行分析,给HIV抗体检测工作提供指导依据。方法采用免疫印迹法(WB)对645例HIV抗体筛查阳性检测样品进行确证检测,对确证结果不确定者进行随访复查。结果 645例筛查阳性者中,确证HIV-1抗体阳性550例,阳性率为85.3%;阴性56例,阴性率为8.7%;不确定结果 39例,占筛查阳性的6%。反应条带gp160和gp120的出现率最高,分别为100%、99.5%;p17和p55出现率较低,为63.5%和40.5%。不确定者中,p24出现单一带型最多占56.4%,7例转为确定者带型分别为gp160/p24、gp160/p24/p66、gp160/p66。结论确定为HIV-1抗体阳性者带型,各带型出现的几率有所差别,HIV抗体不确定者,存在一定的假阳性,应谨慎处理,加强随访检测。
Objective To analyze the positive results of 645 HIV antibody positive screening specimens and provide guidance for HIV antibody testing. Methods Western blotting (WB) was used to confirm the positive test samples of 645 HIV antibody screening tests, and the follow-up was performed on the patients with uncertain confirmation results. Results Of the 645 positive samples, 550 were positive for HIV-1 antibody. The positive rate was 85.3%. The negative rate was 8.7%. The uncertain result was 39, accounting for 6% of the positive results. The bands of gp160 and gp120 were the highest, accounting for 100% and 99.5%, respectively. The incidences of p17 and p55 were lower at 63.5% and 40.5%, respectively. Uncertainty, p24 appeared single band type accounted for up to 56.4%, 7 cases to determine the type were gp160 / p24, gp160 / p24 / p66, gp160 / p66. Conclusion The HIV-1 antibody was positively identified as a banding pattern. The incidence of each band pattern was different. HIV antibody was uncertain and had some false positives. Careful treatment and follow-up testing should be strengthened.