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目的 探讨激活素ⅡA型受体(ActRⅡA)在四氯化碳化学性诱导小鼠急性肝损伤中的表达.方法 将48只清洁级C57BL/6近交系雄性小鼠随机分为2组,分别为对照(橄榄油)组和四氯化碳染毒组,每组24只.采用腹腔注射方式进行一次性染毒,染毒容量为10 ml/kg.检测小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的活力,采用病理形态学方法观察肝组织损伤程度,采用免疫组织化学染色法及实时荧光定量PCR法分别检测肝组织激活素A(ActivinA)和其信号传导分子ActRⅡA蛋白的阳性表达强度及mRNA的表达水平.结果 四氯化碳染毒后7d内小鼠血清ALT、AST的活力和肝损伤面积百分比以及肝脏内Activin A和ActRⅡA蛋白的阳性表达强度和mRNA的表达水平均高于对照组,差异有统计学意义(P＜0.05);且四氯化碳一次性染毒后,随着时间的延长,小鼠血清ALT、AST的活力和肝损伤面积百分比以及肝脏内Activin A和ActRⅡA蛋白的阳性表达强度和mRNA的表达水平均呈下降趋势.结论 ActRⅡA作为激活素的主要受体,其表达升高是介导CCl4诱导急性肝损伤的重要致病因素,阻断其作用可能成为治疗急性肝损伤疾病的有效方法.“,”Objective To explore the expression of activin type Ⅱ receptor (ActRⅡA) and to clarify its role in carbon tetrachloride (CCl4)-induced acute liver injury in mice.Methods Forty-eight male C57BL/6 inbred male mice were randomly assigned to two groups,olive oil solvent control group and CCl4-treated group,24 mice per group.Mice were administrated with CCl4 olive oil solution at 10 ml/kg body weight via intraperitoneal injection once.The level of serum transaminase was measured by biochemical method and histopathological methods were used to observe the degree of liver injury.The mRNA and protein expression of activin A and its signal transduction molecule ActRⅡA were detected by real-time fluorescence quantitative PCR and immunohistochemical staining.Results After CCl4-treatment for seven days,compared with control group,the activities of serum ALT and AST,the injuried liver area percentage,the expression of ActRⅡA protein and mRNA in CCl4-treated group were increased significant with statistically significant difference (P＜0.05).After treatment by CCl4 once,as time Prolongation,the activities of serum ALT and AST,the liver area percentage injuried,the expression of ActRⅡA protein and mRNA in CCl4-treated group were reduced gradually.Conclusion As a major receptor of activin,ActR Ⅱ A is an important pathogen mediating the CCl4-induced hepatic injury in mice.The blockade of expression or action mechanism of ActR Ⅱ A may be an effective target to treatment of liver diseases.