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目的:研究玉叶金花皂苷U对M胆碱能神经支配器官(回肠平滑肌、瞳孔、唾液腺)的影响。方法:1用离体肌收缩力测量装置获取肌收缩力信息。分别往装置浴槽加入阿托品,使其终质量浓度为10 mg·L-1或玉叶金花皂苷U,使其终质量浓度分别为100,50,25 mg·L-1,10 min后开始累积加入溴化乙酰胆碱,观察药物对不同终浓度溴化乙酰胆碱引起肌收缩力的影响,重复试验10次。2小鼠分为正常组和5个造模型组,每组10只,模型组ip毛果芸香碱20 mg·kg-11次,造成M胆碱能神经兴奋模型,15 min后,给成模小鼠尾静脉注射阿托品2.5 mg·kg-1或玉叶金花皂苷U 10,5,2.5 mg·kg-1,并ig碳末悬液,测量ig碳末30 min后小肠碳末推进率。3小鼠分组、造模和给药方法同2,分别于给药后15,60,240 min,测量瞳孔直径和唾液腺分泌量。结果:玉叶金花皂苷U降低肠平滑肌收缩力,使溴化乙酰胆碱终质量浓度与肌收缩力之间的量-效反应曲线右移;玉叶金花皂苷U(5 mg·kg-1)组,小肠碳末推进为(64.20±10.14)%,较模型组降低;给药后15,60,240 min,瞳孔直径分别为(2.5±0.19),(2.2±0.18),(2.1±0.40)mm,较模型组扩大,唾液腺分泌量分别为(102.3±13.57),(90.2±20.51),(68.0±21.52)mg,较模型组减少(P<0.05)。结论:玉叶金花皂苷U能抑制M胆碱能神经兴奋。
Objective: To study the effects of Grifola umbellatus U on the innervation of cholinergic innervation (ileum smooth muscle, pupil, salivary glands). METHODS: 1 Muscle contractility information was obtained with an in vitro muscle contractility measuring device. Atropine was added to the device bath to make the final concentration of 10 mg · L-1 or ginsenoside U, the final concentration was 100,50,25 mg · L-1, 10 min after the cumulative addition of bromine Acetylcholine, observe the effects of different concentrations of drugs on the muscle contractility caused by acetylcholine bromide, repeat the test 10 times. 2 mice were divided into normal group and 5 model group, 10 rats in each group, the model group ip pilocarpine 20 mg · kg-11 times, resulting in cholinergic excitability model of M, after 15 min, Intravenous injection of atropine 2.5 mg · kg-1 or jade leaves Ginsenoside U 10,5,2.5 mg · kg-1, and ig carbon suspension, measured carbon ig 30 min after the small intestine carbon propulsion rate. 3 mice were divided into groups, and the methods of modeling and administration were the same as 2, and pupillary diameter and salivary gland secretion were measured at 15, 60 and 240 min after administration respectively. Results: Jade Ginsenoside U decreased the contractile force of intestinal smooth muscles and shifted the dose-response curve between the final concentrations of acetylcholine (BrdU) and muscle contractility to the right. The concentration of Ginsenoside U (5 mg · kg -1) (64.20 ± 10.14)%, which was lower than the model group. At 15,60,240 min after administration, pupil diameters were (2.5 ± 0.19), (2.2 ± 0.18) and (2.1 ± 0.40) mm, (102.3 ± 13.57), (90.2 ± 20.51) and (68.0 ± 21.52) mg respectively, which were significantly lower than those in model group (P <0.05). Conclusion: Jade Ginsenoside U can inhibit M cholinergic nerve excitability.