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为了进一步探索E类前列腺素(PGE)在免疫调变巨噬细胞系统中可能的作用机理,我们建立了PGE微量放射免疫检测法,应用高比度的~3H-PGE_2以及高度纯化的抗PGE_2抗体,直接定量检测待测样品的PGE含量。此法快速、灵敏、有效、简便。其可测定度在10p8左右,完成整个检测只需4小时左右。此法也可应用于其它种类前列腺素的测定,只须配备相应的抗体以及标准品。体外培养分化成熟的人体激活巨噬细胞仅分泌相当少的PGE,而免疫调变巨噬细胞的PGE分泌量为激活巨噬细胞的50～100倍,提示抑制性巨噬细胞的免疫抑制效应似乎不是PGE介导的。
To further explore the possible mechanism of action of class E prostaglandins (PGEs) in immunomodulatory macrophage system, we established a PGE micro-radioimmunoassay using high specific-3H-PGE_2 and highly purified anti-PGE_2 antibody , Direct quantitative detection of test samples PGE content. This method is fast, sensitive, effective and easy. Its measurability is about 10p8, only about 4 hours to complete the entire test. This method can also be applied to the determination of other types of prostaglandins, only with the appropriate antibodies and standards. In vitro cultured differentiated human activated macrophages secrete only relatively small amounts of PGE, whereas immunopotentiated macrophages exhibit 50- to 100-fold PGE secretion of activated macrophages, suggesting that immunosuppressive effects of suppressive macrophages appear to be Not PGE-mediated.