苯酮尿症(PKU)病人尿中苯丙氨酸(Phe)浓度显著高于正常值。用 HPLC 法测定 Phe 来诊断 PKU 报道较多,但由于所用的UV 检测器灵敏度低,FL 检测器重现性差,均难以满足临床诊断的要求。本文建立的HPLC 柱后光解电化学(EC)联机检测法具有灵敏度高及选择性好等特点,用于定量测定尿中 Phe 的精密度及重现性均良好,能用于PKU 的临床诊断。 Phenylketonuria (PKU) in patients with urine phenylalanine (Phe) concentrations were significantly higher than normal. However, the detection of Phe by HPLC is more and more commonly reported in the diagnosis of PKU. However, due to the low sensitivity of the UV detector used and the poor reproducibility of the FL detector, it is difficult to meet the clinical diagnosis requirements. The established HPLC post-column photolysis electrochemical (EC) on-line detection method with high sensitivity and selectivity and other characteristics for the quantitative determination of urine Phe precision and reproducibility are good, can be used for clinical diagnosis of PKU .