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目的 :研究肉苁蓉总苷 (GCs)对异丙肾上腺素所致小鼠心肌损伤的保护作用。 方法 :采用异丙肾上腺素 (Isoprenaline,ISO)诱发小鼠急性心肌损伤模型。以比色法测定心肌 Se- GSH- Px、SOD活性及丙二醛 (MDA)含量。以生化自动分析仪测血清 CPK活性。电镜检查心肌超微结构的改变。 结果 :ISO(2 0 mg/kg皮下注射连续 2d)使心肌 Se- GSH - Px、SOD活性明显降低 ,MDA含量显著增加 ,并使心肌超微结构严重损伤 ,心肌释放 CPK增加。 GCs(12 5、2 5 0、5 0 0 mg/kg呈剂量依赖性地保护心肌 Se- GSH- Px、SOD活性 ,降低 MDA含量 ,减少 CPK释放 ,减轻 ISO引起的心肌超微结构损伤。 结论 :GCs具有保护心肌损伤和抗脂质过氧化作用
Objective: To study the protective effect of glycosides of cistanche (GCs) on isoproterenol-induced myocardial injury in mice. METHODS: Acute myocardial injury model in mice was induced by Isoprenaline (ISO). The activity of myocardial Se-GSH-Px, SOD, and malondialdehyde (MDA) were measured by colorimetry. Serum CPK activity was measured by a biochemical analyzer. Electron microscopy examined changes in myocardial ultrastructure. RESULTS: ISO (2 mg/kg subcutaneously for 2 consecutive days) significantly decreased the activities of Se-GSH-Px and SOD in myocardium, significantly increased the content of MDA, and severely damaged the ultrastructure of myocardium. Myocardium released CPK increased. GCs (12 5, 250, and 500 mg/kg dose-dependently protected myocardial Se-GSH-Px, SOD activity, decreased MDA content, decreased CPK release, and reduced ISO-induced myocardial ultrastructure damage. : GCs protect myocardial injury and anti-lipid peroxidation