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目的:比较福尔马林固定石蜡包埋组织(FFPET)中3种提取DNA的方法对DNA质量和回收率的影响,探讨一种高效、简便、实用的石蜡组织中DNA提取方法。方法:选取新疆医科大学第一附属医院2004～2007年手术切除的10%甲醛固定石蜡包埋甲状腺癌组织标本,分别以试剂盒法、改良TES水浴脱蜡-酚氯仿法、改良TES水浴脱蜡-试剂盒法提取其DNA,然后进行电泳分析。结果:改良TES水浴脱蜡-酚氯仿提纯DNA法所得样本DNA的OD260/OD280比值为(1.82±0.15),改良TES水浴脱蜡-试剂盒提纯DNA法为(1.86±0.17),两者比较无明显差异(P>0.05);分别与试剂盒法(1.75±0.14)相比,差异有统计学意义(P<0.01)。改良TES水浴脱蜡-试剂盒提纯DNA法、改良TES水浴脱蜡-酚氯仿提纯DNA法所得DNA产量和质量均高于试剂盒法。结论:改良TES水浴脱蜡-试剂盒提纯法简单快捷,是较理想的石蜡包埋组织DNA提纯方法。
OBJECTIVE: To compare the effects of three DNA extraction methods on formalin-fixed paraffin-embedded tissue (FFPET) on DNA quality and recovery, and to explore an efficient, simple and practical DNA extraction method in paraffin tissue. Methods: Tissue samples of 10% formalin-fixed paraffin-embedded thyroid cancer surgically removed from the First Affiliated Hospital of Xinjiang Medical University from 2004 to 2007 were selected and used for the detoxification-phenol-chloroform method and modified TES water bath decontamination - Kit DNA extraction method, and then electrophoresis. Results: The ratio of OD260 / OD280 of the sample DNA obtained by the modified TES water bath dewaxing - phenol chloroform purification was (1.82 ± 0.15) and that of the modified TES water bath decontamination - kit DNA purification was (1.86 ± 0.17) (P> 0.05). Compared with the kit method (1.75 ± 0.14), the difference was statistically significant (P <0.01). Improved TES water bath dewaxing - Kit DNA purification method, modified TES water bath dewaxing - phenol chloroform DNA purification obtained DNA yield and quality are higher than the kit method. Conclusion: Modified TES water bath dewaxing - kit purification method is simple and quick, is an ideal paraffin - embedded tissue DNA purification method.