论文部分内容阅读
在生物体内发生着核酸的合成与降解, 涉及核酸上磷酸二酯键的生成与断裂.磷酸二酯键的断裂模式有氧化性断裂和水解性断裂, 水解性断裂的产物有可能用连接酶再连接. 近年来, 磷酸二酯键水解断裂的配位化学模拟取得了很大进展. 在所发现的具有水解断裂核酸能力的金属配合物中, 有单核金属配合物, 也有双核金属配合物, 涉及的金属离子有过渡金属离子, 也有镧系金属离子, 但反应速率同天然酶相比仍差几个数量级. 用多核金属配合物来模拟核酸酶对磷酸二酯键的水解断裂应当是一个有前途的研究方向.
The synthesis and degradation of nucleic acids occur in organisms and involve the formation and cleavage of phosphodiester bonds in the nucleic acids. The cleavage patterns of the phosphodiester bonds are oxidatively and hydrolytically cleaved, and products of hydrolytic cleavage are likely to be cleaved with ligase In recent years, great progress has been made in the coordination chemistry simulation of the hydrolysis and cleavage of the phosphodiester bond, among the found metal complexes capable of hydrolyzing and cleaving nucleic acids, there are mononuclear metal complexes and dinuclear metal complexes, The involved metal ions are transition metal ions, but also lanthanide metal ions, but the reaction rate is still orders of magnitude lower than that of the native enzyme.Micro-nuclear metal complexes are used to simulate the cleavage of the phosphodiester bond by nucleases Future research direction.