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目的:探讨复方丹参饮对胰岛素抵抗(IR)大鼠血清中细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)的表达。方法:将60只Wistar雄性大鼠适应性的喂养1周,随机分为生理盐水组、胰岛素抵抗组、复方丹参饮预防组(中药预防组)复方丹参饮治疗组(中药治疗组)、罗格列酮组(西药对照组),每组分为12只。采用高脂高糖高胆固醇饮食喂养,建立大鼠IR模型。喂养16周以后采用酶联免疫法检测各组Wistar大鼠血清中ICAM-1、VCAM-1的含量。结果:与生理盐水组比较,除复方丹参饮预防组差异无统计学意义外(P>0.05),其余各组大鼠血清中ICAM-1、VCAM-1的含量均升高(P<0.05),有统计学意义;与胰岛素抵抗组相比较,西药对照组、中药治疗组与中药预防组均能减少大鼠血清中ICAM-1、VCAM-1的含量(P<0.05)。结论:认为复方丹参饮具有抑制大鼠血清血管内皮细胞ICAM-1、VCAM-1的过度表达作用,对胰岛素抵抗大鼠的血管内皮细胞具有保护功效。
Objective: To investigate the expression of ICAM-1 and VCAM-1 in compound insulin resistance (IR) rats. Methods: Sixty Wistar male rats were fed for one week adaptively. They were randomly divided into three groups: saline group, insulin resistance group, compound Danshen Decoction (TCM prevention group), compound Danshen Decoction group (TCM treatment group) Zoster group (western medicine control group), each group of 12. The rats were fed with high-fat, high-sugar and high-cholesterol diet to establish the rat IR model. After 16 weeks of feeding, the contents of ICAM-1 and VCAM-1 in the serum of Wistar rats in each group were detected by enzyme-linked immunosorbent assay. Results: Compared with the normal saline group, the contents of ICAM-1 and VCAM-1 in the serum of the other groups were all increased (P <0.05) except the compound Danshen Decoction prevention group had no significant difference (P> 0.05) (P <0.05). Compared with the insulin resistance group, Western medicine control group, traditional Chinese medicine treatment group and traditional Chinese medicine prevention group could reduce the content of ICAM-1 and VCAM-1 in serum (P <0.05). Conclusion: It is concluded that Fufang Danshen Decoction can inhibit the overexpression of ICAM-1 and VCAM-1 in serum of rat vascular endothelial cells and has the protective effect on the vascular endothelial cells of insulin-resistant rats.