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基于miRNA:mRNA互补原则和CSE cDNA序列,设计了CSE特异的miRNAs.这些CSE特异的miRNAs显著地抑制CSE蛋白表达和H2S产生,增加活性氧的生成,并且诱导人类主动脉平滑肌细胞的增殖.通过构建包含CSE 3′-UTR序列报告基因和抑制荧光素酶活性实验,证实CSE特异的miRNA通过碱基互补结合CSE基因序列.CSE特异的miRNA没有影响其他基因的表达.与CSE特异的siRNAs相比,CSE特异miRNAs在抑制CSE基因表达和H2S的产生方面,展现出显著的高效率.miR-143是一个在心血管组织中高表达的miRNA,下调了CSE基因和其他基因的表达.miR-143抑制H2S的产生,但是对人类主动脉平滑肌细胞的增殖没有影响.本文设计的CSE特异miRNAs为研究调控CSE表达和H2S产生提供一个高效率的工具.这些CSE特异miRNAs具有成为治疗有关氧化应激异常和平滑肌细胞增长相关的心血管疾病的新型药物的潜在可能.
CSE-specific miRNAs were designed based on the principle of miRNA: mRNA complement and CSE cDNA sequence.These CSE-specific miRNAs significantly inhibited CSE protein expression and H2S production, increased reactive oxygen species generation, and induced the proliferation of human aortic smooth muscle cells.Through Constructions containing CSE 3’-UTR sequence reporter and luciferase activity assays confirmed that CSE-specific miRNAs complementarily bind to the CSE gene sequence by base complementation, and that the CSE-specific miRNAs did not affect the expression of other genes.Compared with CSE-specific siRNAs , CSE-specific miRNAs showed markedly high efficiency in inhibiting CSE gene expression and H2S production.miR-143 is a miRNA that is overexpressed in cardiovascular tissues and down-regulates the expression of CSE and other genes.miR-143 inhibits H2S , But had no effect on the proliferation of human aortic smooth muscle cells.The CSE-specific miRNAs designed in this paper provide a high-efficiency tool for the study on the regulation of CSE expression and H2S production.These CSE-specific miRNAs have the potential to be used to treat oxidative stress and smooth muscle Potential for new drugs for cell growth-related cardiovascular disease.