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目的 本文报道 7例脑中枢神经细胞瘤 ,复习文献并探讨其组织学、免疫组化和超微结构特点。方法 复习 1993- 2 0 0 1年间 17例脑室肿瘤 ,进行组织学观察 ,用免疫组化检测几种重要的肿瘤标记物的表达 ,并对部分病例进行电镜观察。通过对PCNA染色和DNA含量图像分析 ,研究本瘤的增殖活性和DNA倍体。结果 17例脑室肿瘤中 ,7例确诊为中枢神经细胞瘤。组织学检查该肿瘤具有一定的形态学特点如由大小一致的圆形细胞组成 ,核圆形 ,点彩状 ,细胞核周有明显的空晕 ,可见神经纤维小岛等。免疫组化结果显示全部病例均为Syn阳性。电镜观察可见较多的神经内分泌颗粒及含微管结构的突触。PCNA染色结果显示肿瘤细胞增殖指数 <5 %。DNA含量分析显示 ,7例中枢神经细胞瘤平均 78%为 2倍体细胞 ,提示为良性肿瘤。结论 中枢神经细胞瘤具有一定的组织学特征以及免疫组化和电镜特点 ,这些特点有助肿瘤的正确诊断
Objective This paper reports 7 cases of brain central neuroblastoma, review the literature and explore its histological, immunohistochemical and ultrastructural features. Methods Twenty-seven cases of ventricular tumors were reviewed from 1993 to 2001, histological observation was performed, and the expression of several important tumor markers was detected by immunohistochemistry. Electron microscopy was also performed on some cases. The proliferative activity and DNA ploidy of this tumor were studied by analyzing PCNA staining and DNA content. Results Of the 17 cases of ventricular tumors, 7 were diagnosed as central neuroblastoma. Histological examination of the tumor has a certain morphological characteristics, such as the same size by the circular cells, nuclear round, point color, the nucleus was obviously empty halo, visible nerve fiber island. Immunohistochemical results showed that all cases were Syn positive. Electron microscopy showed more neuroendocrine granules and microtubule-containing synapses. PCNA staining showed tumor cell proliferation index <5%. DNA content analysis showed that 7 cases of central neuroblastoma average of 78% of diploid cells, suggesting that benign tumors. Conclusions Central neurocytoma has certain histological features as well as immunohistochemical and electron microscopic features that contribute to the correct diagnosis of tumors